Intended use:
RIDASCREEN® Sulfamethazin is a competitive enzyme immunoassay for the quantitative analysis of sulfamethazine in milk, meat and kidney and for the qualitative analysis of sulfamethazine in meat and kidney (not from rabbit).
General information:
Sulfonamides are widely used as feed additives, mainly for fattening of calves and
pigs. Combined with inhibitors of dihydrofolate reductase such as trimethoprim,
tetroxoprim, or pyrimethamine sulfonamides are also used in veterinary medicine for the treatment of intestinal infections, mastitis, pulmonitis and other (systemic) diseases. Sulfonamide residues may therefore occur in food of animal origin such as meat and milk. Particularly the transmission of the carcinogenic sulfamethazine represents a threat to human health. According to the EU-Law a maximum residue limit for all substances of the sulfonamide-group of 100 ppb in muscle, fat, liver and kidney and of 100 ppb in milk is valid.
Specifications:
Format:Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Standards:0 ppb (zero standard), 1 ppb, 3 ppb, 9 ppb, 27 ppb,
81 ppb
Sample preparation: milk: after 1:10 dilution direct use in the assay
meat and kidney (quantitative): extraction, centrifugation, evaporation and degreasing
meat and kidney (qualitative): homogenization and
centrifugation
Incubation time:2 h 30 min
Detection limit: Quantitative results - Detection limit (corresponding to the standard substance):
milk: approx. 10 ppb
meat and kidney: approx. 2 ppb
Qualitative results - Cut off:
meat and kidney (not from rabbit): 100 ppb
Cross-reactions: Sulfamethazine: 100 %
Sulfamerazine: approx. 56 %
Sulfamoxol: approx. 2 %
All further sulfonamides: < 1 %
