Intended use:
RIDASCREEN® Nitrofuran (AOZ) is a competitive enzyme immunoassay for the quantitative analysis of AOZ in shrimps, meat (chicken, pork, beef), liver, fish, whole egg and milk.
General information:
Nitrofurans are synthetic broad-spectrum antibiotics, which are frequently employed in animal production for its excellent antibacterial and pharmacokinetic properties.
They had been also used as growth promoters in shrimps, poultry and pig production. In long term studies with experimental animals the parent drugs and their metabolites showed carcinogenic and mutagenic characteristics. This has led to a prohibition of nitrofurans for the treatment of animals used for food production.
The nitrofuran drugs furaltadone, nitrofurantoin and nitrofurazone were banned from
use in food animal production in the EU in 1993, and the use of furazolidone was
prohibited in 1995.
The analysis of residues of nitrofuran drugs needs to be based on the detection of
the tissue bound metabolites of the nitrofuran parent drugs. Since the parent drugs
are very rapidly metabolized, they are not detectable shortly after treatment. The
tissue bound nitrofuran metabolites are detectable for a long time after administration
and therefore they are used for the detection of the abuse of nitrofurans. Nitrofuran metabolites are found after administration of Furazolidone (metabolite: 3- amino- 2-oxazolidinone = AOZ), Furaltadone (metabolite: 3-amino-5-morpholinomethyl-
2-oxazolidinone = AMOZ), Nitrofurantoin (metabolite: 1-aminohydantoin = AHD) and Nitrofurazone (metabolite: semicarbazide = SEM).
Specifications:
Format: Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Standards: 0 ppt (zero standard), 25 ppt, 50 ppt, 100 ppt, 200 ppt, 400 ppt
Sample preparation: homogenization (milk samples: precipitation), derivatisation, extraction, centrifugation, evaporation and
degreasing
option I (short):
sample preparation part 1: approx.1,5 h
incubation: 3 h
sample preparation part 2: approx. 1,5 h
option II (long):
sample preparation part 1: approx.1,5 h
incubation (over night): approx. 16 h
sample preparation part 2: approx. 1,5 h
Incubation time: 1 h 15 min
Detection limit: shrimps, fish, milk: approx. 50 ppt
meat, liver, whole egg: approx. 100 ppt
Cross-reactions: AMOZ: < 0.01 %
AHD: < 0.01 %
SEM: < 0.01 %
Note: For the determination of AMOZ we refer to the RIDASCREEN® Nitrofuran (AMOZ) test with
Art. No. R3711.
