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RIDASCREEN® Melengestrolacetat

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R6502 RIDASCREEN® Melengestrolacetat Microtiter plate with 48 wells (6 strips with 8 re call for price none

Intended use:
RIDASCREEN® Melengestrolacetat is a competitive enzyme immunoassay for the quantitative analysis of melengestrol acetate in bovine perirenal fat and muscle meat.
The performance of the test is adjusted to the current legal demands and well suited for the control of meat exported by the USA and Canada.

General information:
Synthetic gestagens can be used for estrus inhibition or synchronisation after
break off and improvement of fertility. In addition one of these substances,
melengestrol acetate (MLGA), is a licensed growth promoting feed additive for
heifers in the USA and Canada. The admitted dose is 0.5 mg per day and head,
given as a feed premix. Its mode of anabolic action is unclear, but it stimulates the ovarian synthesis of the endogenous anabolic steroid estradiol and that may have androgenic side effects. MLGA belongs to the most active synthetic gestagens. Its oral bioactivity is about 10 or 100 times higher than the activities of the gestagens chlormadinone acetate (CMA) or medroxyprogesterone acetate (MPA), resp. If given parenterally the hormonal activity of MLGA is still 125 times higher than the one of progesterone. Due to its strongly lipophilic properties, MLGA is accumulated in fat 200-fold higher than in blood plasma.
In the European Union the use of sexual hormones for growth promoting purposes
is generally forbidden since 1988, and also the import of meat from hormone treated cattle into the EU is prohibited.

Format:Microtiter plate with 48 wells (6 strips with 8 removable wells each)
Standards:0 ppb (zero standard), 0.15 ppb, 0.45 ppb, 1.35 ppb,
4.05 ppb, 12.15 ppb
Sample preparation: extraction over night
centrifugation, evaporation, freezing, centrifugation and chromatographic purification with RIDA® C18 column
(Art. No.: R2002)
Incubation time:2 h 30 min
Detection limit: bovine perirenal fat: 0.3 ppb
muscle meat: 0.075 ppb
Cross-reactions: Melengestrol acetate (MLGA): 100 %
Megestrol acetate (MGA): approx. 10 %
Medroxyprogesterone acetate (MPA): approx. 6 %
6α-Methyl-17α-OH-Progesterone acetate: approx. 5 %
Chlormadinone acetate (CMA): approx. 5 %
17α-Acetoxyprogesterone: approx. 4 %
16α-Methylprogesterone: 0.0035 %
cross reaction of all other substances being checked in the assay is less 0.003 %