Recommended for purification of proteins containing His-tag sequences. A specially formulated cocktail of five protease inhibitors with broad specificity for the inhibition of cysteine, serine, aspartic, thermolysin-like proteases and aminopeptidases. When reconstituted to 1ml, each vial will contain 100 mM AEBSF.HCl, 5 mM bestatin, 1.5 mM E-64, 2 mM pepstatin A and 200μM phosphoramidon, disodium salt. One vial is recommended for about 10 g of cells. For applications where the use of DMSO is not recommended.