Recommended for use with mammalian cell and tissue extracts. A specially formulated cocktail of six protease inhibitors with broad specificity for the inhibition of aspartic, cysteine and serine proteases, as well as, aminopeptidases. Each vial contains 100 mM AEBSF.HCl, 80μM aprotinin recombinant (animal-free), 5 mM bestatin, 1.5 mM E-64, 2 mM leupeptin hemisulfate and 1 mM pepstatin A. One ml is recommended for the inhibition of proteases extracted from 20 g of tissue. For applications that require animal-free reagents.