A cocktail of five protease inhibitors which has been optimized for the inhibition of a broad range of proteases and esterases. 1X stock solution contains 500 μM AEBSF.HCl, 150 nM Aprotinin, recombinant (animal-free), 1μM E-64, 0.5 mM EDTA and 1μM leupeptin hemisulfate. For applications that require animal-free reagents.