DON Immunoaffinity
Column (IAC)
Cat.# DONIAC
Intended use
The application of IAC on the complicated sample matrix will facilitated the purification of DON in food and feed , After this step, the DON can be later quantitative analysis by TLC, HPLC ,GC ,LC-MS/MS, EIA.
Capacity: above 500 ng of DON
Storage Conditions: IAC should be stored at 2-8℃. Do not freeze.
Use
Clean up of sample extracts for further analysis.
summary
DON main ingredient for DON (deoxynivalenol fusarium enol ),belonging to a single trichothecenes compound, DON is common fungal toxin in the food , widesperad in nature, because they have a high cytotoxic and immunosuppressive nature, therefore, constitute a threat to human and animal health ,in particular has a significant on the immune function also posing a great threat on human health structure, DON is classified as a category 3 carcinogen, Countries in the world have strict limits on DON in food, feed.
Use Principles
The IAC is based on the reaction between antigen and antibody. This DON IAC contains gel binding with. The DON in the samples will be extracted, filtered, diluted and passed through the DON IAC.DON in the extract will be retained in the IAC by the DON specific antibody, the impurity in the extract will pass through. After rinsing to completely remove the unwanted matrix, the DON is eluted by Methanol by de-associating the DON from the antibody. This elute will be ready for instrumental analysis.
Materials Provided
25 disposable IAC per box, 1ml each.
Instructions.
Precautions
DON are toxic substance. Please wear gloves to go experimental operation.
Used lab wares and DON solution preferably be soaked with 5% sodium hypochlorite solution soak over night.
Do not use the reagents after the expiration date.
IAC stored at 2-8℃，no not freeze
Reagents should be brought to room temperature (22-25℃) prior to use.
Operation Procedure
Grind the sample in a blender and sieve through # 20 sieve. Sample will not be extracted right away should be store in the refrigerator.
Weigh 10 gram of sample and add 10 ml of water.
Homogenize for second minute or shake for 30 minute.
Filter this diluted extract through a microfiber filter, load 1ml of this clear solution onto the IAC.
Connect syringe barrel reservoir to the column
Load the column with 1ml diluted and filtered extract and control the flow rate at 1-2 drops per second. Discard the pass through liquid
Use 5 ml of water to rinse the column and discard the rinse.
Press the air into the column to blow out all the liquid
Use 1 ml of methanol to elute the sample (flow at one drop/2-3 seconds).
collect the sample for further testing.
