Size: 100 µl
Description
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq]
Recommended Applications
WB
Applications and Recommended Dilution Factors
WB: 1:100-1:1000
Species Reactivity *
Human, Mouse
*Other species have not been tested
Products Data
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Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
MECP2 antibody
diluted at 1:1000
|
Sample(30 ug of whole cell lysate)
A:HeLa S3
B:Hep G2
10% SDS PAGE
diluted at 1:200
|
Specificity
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 202 of MECP2
Storage Condition and Buffer
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Purified by antigen-affinity chromatography.
Alternative Names
MECP2 antibody, AUTSX3 antibody, MRX16 antibody, MRX79 antibody, MRXS13 antibody, MRXSL antibody, PPMX antibody, RS antibody, RTS antibody, RTT antibody, Methyl-CpG-binding protein 2 antibody
