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Water-sensitive paper 76X26mm

Price(USD):$86.71

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Equl SKU Product Name UOM Specification Units in Stock Price(USD) Add to Cart:
WSP76X26 Water-sensitive paper 76X26mm one pack contains 50 stripes the size of stripe is 76x26mm 26 $86.71  
Introduction:

Special paper which discolours blue by contact with aqueous drops

Used for visible control of aqueous droplets or spray distribution

Aqueous droplets on it turn dark blue immediately

Accurate drop size and pattern measurements

Stored in sealed packages

2 sizes available

one pack contains 50 stripes, the size of stripe is 76x52mm

WSP is a special slide-strip serving as a visible control of spray distribution, which discolours by contact with aqueous drops. It is covered with a yellow surface on the upper side serving as a visible control of the array quality.
Spotting aqueous sample droplets on it, this water-sensitive surface is turning dark blue immediately.

It has been also developed for the quick and easy control of microarray spotting process and detection of array defaults. It helps to visualize droplet distributions, droplet densities and array quality. WSP should always be used prior to spotting to detect any array defaults. To evaluate array quality, just place the WSP instead of a microarray slide on the micro arrayer. Spot the array according to the array pattern, remove the strip and cover it with adhesive scotch-tape. Then check the droplet pattern or compare it with the default array.

Handling, Limitations and Storage
To ensure a constant quality, the WSP is packaged in a dust-safe bag and sealed under inert gas. Sealed WSP is stable for at least 12 months if stored at room temperature. Unsealed packages, which are not used immediately, should be stored dry at room temperature in a dust-safe bag.

Analysis and Evaluation Check the droplet pattern
For a quick estimate compare the exposed droplets with a known standard or examine the droplets either using a hand lens or an automatic image analyzer. Droplet counting by hand lens on WSP is limited to a maximum of around 600 droplets per cm2. For higher droplet densities use automatic image analyzer. Accurate drop size and pattern measurements with commercial imaging software can only be made if the drop density is low enough for the majority of droplets not to overlap each other.

WSP is produced under careful and rigid process control. To maintain this high quality standard, follow the instruction below.

Use always gloves (polyethylene or rubber) when handling the WSP to avoid any staining and contamination of the water-sensitive surface.

The WSP should be processed under clean conditions only. Dust particles may cause local alterations in the wetting characteristics of the slide surface, which result in depositing failures.

Since the yellow surface is sensitive, direct contact and any touch of the spotted slide surface must be avoided.

The WSP cannot be used at a relative humidity of more than 80 %. Although it turns light blue at a relative humidity of 80 % the stains produced by droplets still show sufficient contrast to allow visual control beyond this limit.

If you want to produce high-density arrays with a spot size smaller than 100 µm you should adjust the relative humidity to approximately 40 - 50 %. Pure water stains the WSP in drop size as small as 70 µm at room temperature and relative humidity of 30 %.

WSP is not recommended for use at temperatures below 10 °C.

After spotting the WSP must be protected with a colourless, thin adhesive foil (e.g. scotch tape). Avoid air pockets while adhering the tape.

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